NUCLEOSIDE TRIPHOSPHATE PHOTOAFFINITY PROBE, METHOD FOR PREPARING SAME, AND APPLICATIONS THEREOF
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United States of America
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Issued Date -
Dec 5, 2024
app pub date -
Jun 3, 2024
filing date -
Jun 3, 2024
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Abstract
A nucleoside triphosphate photoaffinity probe, method for preparing same, and applications thereof. The nucleoside triphosphate photoaffinity probe is a novel small molecule activity probe for detecting nucleoside triphosphate-binding proteins and is based on the structure of nucleoside triphosphates (GTP and ATP) connected to a smaller photoaffinity side chain modification. This probe can effectively label nucleoside triphosphate-binding proteins in cell lysates for high-throughput proteomics analysis, identify and analyze the binding sites of the probe, and can also be used to analyze the action sites of nucleoside triphosphate competitive inhibitors, thus having significant practical application value.
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