DETECTION OF TARGET NUCLEIC ACID SEQUENCE BY USING SYNTHETIC NON-NATURAL BASE-BEARING TAG OLIGONUCLEOTIDE
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United States of America
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Nov 28, 2024
app pub date -
Sep 16, 2022
filing date -
Sep 16, 2022
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Abstract
A method and composition for the detection of a target nucleic acid sequence using a tag oligonucleotide that contains a synthetic unnatural base. The protocol of the present disclosure increases a target signal by improving the binding affinity or specificity of the hybridization between the target-dependent fragment and the fragment-hybridizing oligonucleotide, and thus it is possible to detect the target sequences with more improved sensitivity.
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