HUMAN BONE MARROW-DERIVED MESENCHYMAL STEM CELL SHEETS AND METHODS FOR THEIR PRODUCTION
Number of patents in Portfolio can not be more than 2000
United States of America
Stats
-
N/A
Issued Date -
Nov 28, 2024
app pub date -
Sep 30, 2022
filing date -
Sep 30, 2022
priority date (Note) -
Published
status (Latency Note)
Importance
Abstract
The disclosure provides a human bone marrow-derived mesenchymal stem cell (hBMSC) sheet comprising one or more layers of human hBMSCs, wherein the cell sheet is prepared from a human clonal bone marrow-derived mesenchymal stem cell line generated from a single cell. The disclosure also provides methods for producing human bone marrow-derived mesenchymal stem cell sheets comprising culturing hBMSCs in culture solution on a temperature-responsive polymer which has been coated onto a substrate surface of a cell culture support, wherein the temperature-responsive polymer has a lower critical solution temperature in water of 0-80° C.; adjusting the temperature of the culture solution to below the lower critical solution temperature, whereby the substrate surface is made hydrophilic and adhesion of the cell sheet to the surface is weakened; and detaching the cell sheet from the culture support. The cell sheet may be treated with interferon gamma (IFN-γ) or basic fibroblast growth factor (bFGF).
First Claim
Family
International Classification(s)
- [Classification Symbol]
- [Patents Count]
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully