SUGAR CHAIN ANALYSIS METHOD
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United States of America
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Nov 21, 2024
app pub date -
Jun 28, 2022
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Jun 28, 2022
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Abstract
A sugar chain analysis method is a method for analyzing a sugar chain structure bound to an antibody, the method including: a releasing step of adding a sugar cleavage enzyme to a sample containing an antibody, so that a sugar chain bound to the antibody is specifically released to obtain a free sugar chain; a recovery step of recovering the free sugar chain by affinity purification; a hydrophobization step of hydrophobizing the free sugar chain to obtain a hydrophobized sugar chain; and a measurement step of performing supercritical liquid chromatography and tandem mass spectrometry in this order on the hydrophobized sugar chain.
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