Transcription Mediated Amplification Methods for RNA Detection
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United States of America
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Nov 21, 2024
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Aug 5, 2024
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Aug 5, 2024
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Abstract
Provided herein are methods for identifying antibiotic resistance in a subject in need thereof and for detecting drug resistance in a blood-borne pathogenic bacteria. The methods amplify RNA isolated from whole blood in a multiplexed, isothermal nucleic acid sequence-based amplification (NASBA) reaction using at least one isothermal primer pair with nucleotide sequences specific to a drug resistance marker and with a labeled detector probe binding site and a T7 promoter sequence. Amplicons are hybridized to drug resistance marker probes and to a labeled universal detector probe. A signal indicates drug resistance in the blood-borne pathogenic bacteria.
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