RHODOPSEUDOMONAS PALUSTRIS TIE-1 STRAINS AND METHODS FOR BIOPLASTIC PRODUCTION
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United States of America
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Nov 21, 2024
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May 16, 2024
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May 16, 2024
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Abstract
Transgenic strains of R. palustris TIE-1 and methods of use thereof for bioplastic production are provided. An exemplary transgenic R. palustris TIE-1 microorganism includes an exogenous RuBisCo ΩrubI gene; and an exogenous RuBisCo ΩrubII gene. An exemplary method of producing the bioplastic polyhydroxybutyrate (PHB) includes culturing a transgenic R. palustris TIE-1 microorganism in a culture medium, the transgenic R. palustris TIE-1 microorganism comprising an exogenous RuBisCo ΩrubI gene; and an exogenous RuBisCo ΩrubII gene; such that the exogenous RuBisCo ΩrubI and ΩrubII genes are overexpressed; and recovering the PHB from the culture medium.
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