Importance

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Abstract

The present invention provides a means for producing a peptide with a small number of steps in a short time. One aspect of the present invention relates to a method for producing a peptide, comprising: a C-terminal activation step of introducing an N-terminally protected amino acid or peptide, in which an N-terminal amino group is protected by an N-terminal protecting group, and a C-terminal activator into a flow reactor, thereby activating a C-terminal carboxyl group of the N-terminally protected amino acid or peptide in the flow reactor to form a C-terminally activated derivative of the N-terminally protected amino acid or peptide; a condensation step of introducing the C-terminally activated derivative of the N-terminally protected amino acid or peptide and a C-terminally protected amino acid or peptide, in which a C-terminal carboxyl group is protected by a tag protecting group, into a flow reactor, thereby condensing the N-terminally protected amino acid or peptide with the C-terminally protected amino acid or peptide on the N-terminal side in the flow reactor to form an N-terminally and C-terminally protected peptide extended N-terminally; an N-terminal deprotection step of introducing the N-terminally and C-terminally protected peptide extended N-terminally and a deprotecting agent for an N-terminal protecting group into a flow reactor, thereby removing the N-terminal protecting group for deprotection in the flow reactor to form a C-terminally protected peptide extended N-terminally; and an extraction step of mixing a reaction mixture containing the C-terminally protected peptide extended N-terminally with an aqueous phase to extract the C-terminally protected peptide extended N-terminally into an organic phase. Another aspect of the present invention relates to an apparatus for producing a peptide.

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First Claim