RAPID EXPRESSION AND PURIFICATION OF THERMOSTABLE PROTEINS INCLUDING TAQ POLYMERASE
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United States of America Patent
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Issued Date -
Oct 24, 2024
app pub date -
Aug 11, 2022
filing date -
Aug 11, 2022
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Abstract
Genetically modified microorganisms enabling the simple, rapid production and purification of thermostable polymerases are described. Methods of rapid purification of a thermostable polymerase are described including the steps of autoinducible expression achieving high titers of soluble protein; autolysis of cells post harvest, autohydrolysis of host nucleotides through expression of a thermolabile endonuclease; and heat denaturation and precipitation of contaminating proteins. Polymerases are obtained with >95% purity, and are readily usable in standard PCR.
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