METHOD OF IDENTIFYING SPLICED PEPTIDE
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United States of America Patent
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Issued Date -
Oct 17, 2024
app pub date -
Jul 22, 2022
filing date -
Jul 22, 2022
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Abstract
A method of identifying an unknown spliced peptide derived from a known parent peptide contained in a biological sample, the method comprising: (1) a first step involving performing mass spectrometry on the sample to obtain mass spectrometry data regarding a peptide contained in the sample; (2) a second step involving searching a primary library, which is a database of known amino acid sequences, for an amino acid sequence that matches the mass spectrometry data, to identify a parent peptide contained in the sample, (3) a third step involving creating a secondary library including a candidate group of spliced peptides obtainable from the identified parent peptide; and (4) a fourth step involving searching the secondary library for an amino acid sequence that matches the mass spectrometry data, to identify a spliced peptide contained in the sample.
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