SYSTEMS AND METHODS FOR FLUORESCENCE MICROSCOPY CHANNEL CROSSTALK MITIGATION
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United States of America Patent
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Issued Date -
Oct 3, 2024
app pub date -
Jun 6, 2023
filing date -
Mar 31, 2023
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Methods and system are provided herein for crosstalk mitigation, such as the removal of crosstalk interference from a microscopy image signal to recover an intended image from the image signal. In one example, a method includes accumulating a plurality of weighting constant values and variance data to determine a weighting constant of a weighted interference signal of a microscopy image, wherein determining the weighting constant comprises fitting the plurality of weighting constant values and variance data to a quadratic curve; determining the weighted interference signal of the microscopy image based on the weight constant; removing the weighted interference signal from an overall image signal of the microscopy image; and determining a recovered image signal based on removal of the weighted interference signal from the overall image signal.
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