METHODS AND COMPOSITIONS FOR QUANTITATION OF PROTEINS AND RNA
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United States of America Patent
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Issued Date -
Oct 3, 2024
app pub date -
Mar 21, 2023
filing date -
Jan 13, 2020
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Abstract
Disclosed herein include systems, methods, compositions, and kits for determining expressions of proteins and genes simultaneously, and for sample indexing. The method can comprise extending a cellular component-binding reagent specific oligonucleotide hybridized to an oligonucleotide barcode to generate an extended cellular component-binding reagent specific oligonucleotide. The extended cellular component-binding reagent specific oligonucleotide can be separated from the oligonucleotide barcodes. The separated extended cellular component-binding reagent specific oligonucleotide can be amplified separately from barcoded cDNA.
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