GLUCOAMYLASE ENGINEERED YEAST AND FERMENTATION METHODS
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United States of America Patent
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Issued Date -
Oct 3, 2024
app pub date -
Feb 9, 2024
filing date -
Feb 28, 2018
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Abstract
The invention is directed to an engineered yeast including an exogenous nucleic acid encoding a glucoamylase comprising SEQ ID NO:1 and SEQ ID NO:4, or a variant thereof. The engineered yeast are able to provide glucoamylase into a fermentation media and cause degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. High titers of bioproduct (e.g., 70 g/kg of ethanol) can be achieved, along with low residual glucose levels. Further the yeast exhibit good growth and bioproduct product at temperatures of 32° C. or greater.
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