TETRAZOLIUM-BASED COLORIMETRIC ASSAY
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United States of America Patent
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Sep 19, 2024
app pub date -
Mar 11, 2024
filing date -
Mar 14, 2023
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Abstract
Colorimetric based high-throughput assay systems are described that utilize colorimetric agents that exhibit both high water solubility characteristics in both an oxidized and a reduced state and exhibit intense UV/vis absorption in only one of the oxidized or reduced states, with absorption in the other state being either non-existent or very low. Disclosed assays are useful in examining the efficacy of enzyme inhibitors, including helicase or kinase inhibitors.
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