SIMULTANEOUS MULTI-SPECIES SUPER-RESOLUTION IMAGING VIA TEMPORAL MULTIPLEXING AND SINGLE- PHOTON DETECTOR ARRAY
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United States of America Patent
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Sep 5, 2024
app pub date -
Jun 29, 2022
filing date -
Jun 29, 2021
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Abstract
A laser scanning microscope configured to illuminate a sample with a plurality of pulsed excitation light beams having different excitation spectral components is provided. The laser scanning microscope has a single-photon detector configured to detect a fluorescence signal emitted by the sample, the fluorescence signal having different spectral components, an excitation spectrum encoder configured to impose a respective time delay on each excitation spectral component, an emission spectrum encoder configured to impose a respective time delay on each emission spectral component, and a multi-species decoder configured to decode the excitation spectrum, the emission spectrum and a fluorescence decay curve for the fluorescent species contained in the sample.
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