DETERMINATION OF CELLULAR KINETICS AND BIODISTRIBUTION FOR CAR-T CELLS
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United States of America Patent
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Issued Date -
Aug 22, 2024
app pub date -
Oct 12, 2021
filing date -
Oct 12, 2020
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Provided herein, among other things, is a method of determining a transgene copy number in a biological sample, the method comprising: (a) extracting genomic DNA (gDNA) from the biological sample using an automated process, thus isolating gDNA; (b) performing digital droplet PCR (ddPCR) on the isolated gDNA using one or more primers to amplify a transgene of interest and to amplify a reference gene, thus normalizing gDNA input; and (c) determining transgene copy number in the biological sample.
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