Preparation Method and Enrichment Method for Cyanidin-3-Diglucoside-5-Glucoside
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United States of America Patent
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Issued Date -
Aug 22, 2024
app pub date -
Dec 21, 2023
filing date -
Feb 17, 2023
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Abstract
The present disclosure discloses a preparation method and enrichment method of cyanidin-3-diglucoside-5-glucoside, which relates to the technical field of biochemical industry. Enzyme is used to catalyze the substrate to obtain cyanidin-3-diglucoside-5-glucoside. The temperature of the catalytic reaction is 20-40° C., and the reaction time is 10-30 h. This enzyme can convert various anthocyanins into cyanidin-3-diglucoside-5-glucoside, thereby increasing the content of target anthocyanin. The method is simple to operate, specifically improves the content of cyanidin-3-diglucoside-5-glucoside in the crude extract, and reduces the types of anthocyanins in the crude extract to a certain extent, thereby reducing difficulty of subsequent separation and purification. The use of organic reagents and equipment in the traditional enrichment and purification process is reduced, which is energy-saving and environmentally friendly, and the product obtained by catalysis is of good quality, high yield and high purity.
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