MANUFACTURING METHOD OF CELLULAR STRUCTURES
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United States of America Patent
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Aug 22, 2024
app pub date -
Apr 17, 2024
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Jun 26, 2015
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Abstract
The present disclosure aims to provide a manufacturing method of cellular structures. The manufacturing method comprises: preparing a cell culture container comprising: a culture surface coated with a cell non-adhesive material; and a plurality of first coated regions formed by coating the culture surface with a temperature-responsive polymer or a temperature-responsive polymer composition, wherein each of the first coated regions has a surface zeta potential of 0 mV to 50 mV; seeding cells on the culture surface; culturing the cells in a medium; performing medium replacement; and culturing the cells adhered to the first coated regions to form cellular structures.
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