CULTURE METHOD FOR CELLS AND PRODUCTION METHOD FOR USEFUL SUBSTANCE
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United States of America Patent
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Issued Date -
Jul 18, 2024
app pub date -
Mar 29, 2024
filing date -
Sep 30, 2021
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Abstract
An object of the present invention is to provide a culture method for cells, which makes it possible to suppress clogging of a filtration filter flow channel in a case where cells are cultured at a high density, and a production method for a useful substance using the culture method for cells. According to the present invention, there is provided a culture method for cells, which includes a culture step of culturing a cell suspension having a cell density of 0.7×108 cells/mL or more in a culture tank while introducing a gas into a culture solution and a membrane separation treatment step of passing a cell suspension extracted from the culture tank through a separation membrane to separate the cell suspension into a cell-containing liquid and a permeated liquid, where in the culture method for cells, an anti-foaming component to be added to the culture tank is such that an adding amount thereof per unit culture solution amount and per unit time is 0.70 mg/hour/L or less.
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