METHODS FOR PRODUCING INTERMEDIATE ANTIBODIES REGIOSELECTIVELY MODIFIED, AND ANTIBODY DERIVATIVES REGIOSELECTIVELY HAVING BIOORTHOGONAL FUNCTIONAL GROUP OR FUNCTIONAL SUBSTANCE
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United States of America Patent
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Issued Date -
Jul 4, 2024
app pub date -
Mar 7, 2024
filing date -
Sep 8, 2021
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Abstract
A method for producing an intermediate antibody regioselectively modified, which comprises:
- (1) mixing a solution comprising an antibody material and a solution comprising a reagent for regioselectively modifying an antibody in a micromixer to form a mixture comprising the antibody material and the reagent,wherein the reagent comprises a compound comprising an affinity substance to an antibody and a reactive group to an antibody; and(2) passing the mixture through a reaction channel to allow the antibody material and the reagent to react in the reaction channel to form a solution comprising an intermediate antibody regioselectively modified,wherein the processes (1) and (2) are continuously performed in a flow microreactor,is capable of rapidly producing a desired antibody derivative regioselectively having a functional substance or functional substances.
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