FAST MULTIPHOTON MICROSCOPE
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United States of America Patent
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Issued Date -
Jun 27, 2024
app pub date -
Jan 31, 2020
filing date -
Feb 1, 2019
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Abstract
The invention provides improved systems and methods for multiphoton microscopy including pixel clocking techniques for minimizing pixel integration time and providing consistent signal intensity with maximized imaging speeds. Various systems and method are described for optimizing laser repetition rate based on dye lifetime, combining polygonal mirror scanning and stage translation, using the laser pulse signal to time pixel collection, and minimizing laser pulses and dye usage based on signal to background ratios.
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