SYSTEMS AND METHODS FOR PREPARING A POLYPEPTIDE FROM A MIXTURE
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United States of America Patent
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Issued Date -
Jun 27, 2024
app pub date -
Dec 15, 2023
filing date -
Jul 2, 2018
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Abstract
Embodiments of the present disclosure are directed to methods for preparing a target polypeptide from a mixture including the target polypeptide. The method may include contacting the mixture to a hydrophobic interaction chromatography (HIC) apparatus including multiple chromatographic zones. The method may further include passing the target polypeptide through the outlets of at least a first zone and a second zone of the HIC apparatus. A residence time for the mixture including the target polypeptide in a first zone may be approximately the same as a residence time of one or more mobile phases in the second zone.
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