CONSTRUCTION METHOD AND APPLICATION OF OVARIAN GRANULOSA CELL LINE OF ONCORHYNCHUS MYKISS
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United States of America Patent
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May 23, 2024
app pub date -
Nov 17, 2023
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Nov 18, 2022
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Abstract
A construction method and an application of an ovarian granulosa cell line of Oncorhynchus mykiss are provided. The construction method includes transferring a single follicle to a complete Minimum Essential Medium (MEM) containing collagenase H for digestion and transferring primary ovarian granulosa cells to 18° C. for constant temperature culture. The disclosure also provides an ovarian granulosa cell line constructed according to the construction method, and the application of the ovarian granulosa cell line in the separation and culture of fish granulosa cells, the molecular regulation mechanism of granulosa cells and the establishment of hormone metabolism cell models. The construction method of the disclosure has strong repeatability and simple operation steps, and after primary culture, the granulosa cells have good growth state and stable physiological state.
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