Use of Biological Enzyme for Preparing Orlistat Intermediate, and Preparation Method
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May 16, 2024
app pub date -
Jan 6, 2021
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Jan 7, 2020
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Provided is a method for biosynthesis of (R)-β-hydroxytetradecanoate compounds. The method comprises the following steps of: reacting β-carbonyl tetradecanoate in a ketoreductase and a glucose dehydrogenase or a fusion enzyme thereof, glucose, NADP+ and a buffer solution to obtain (R)-β-hydroxytetradecanoate compounds. The method is an enzyme-catalyzed biosynthesis method having simple operations, conventional equipment and an environmentally friendly process, and the prepared product has high purity, high yield and a high ee value.
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