MEMBRANE PROTEIN INTERACTION SCREENING PLATFORM BASED ON CELL-CELL ADHESION EFFECTS
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United States of America Patent
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Issued Date -
May 9, 2024
app pub date -
Jan 2, 2024
filing date -
Jul 1, 2021
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Abstract
The present application relates to the field of cell immunity, and discloses an interacting protein screening platform for preparing a cell membrane. A cell membrane protein and a membrane protein encoding gene to be tested are respectively expressed in different cells; after staining, cells expressing different membrane proteins and cells expressing target proteins are co-incubated; and then interacting membrane proteins are obtained by screening, and specific sequences thereof are determined by sequencing. The screening platform provided in the present application can screen a variety of cell membrane proteins and membrane protein-based engineering cells, and has strong specificity, thereby greatly improving the efficiency of screening interacting proteins and reducing the difficulty, providing a simple and convenient tool for studying the mechanism of action of cell membrane proteins, and improving the screening throughput.
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