IMMUNE REPERTOIRE MINING
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United States of America Patent
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May 9, 2024
app pub date -
Oct 19, 2023
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Apr 12, 2016
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Abstract
The present invention provides a method for producing encapsulated natively-paired scFv amplicons, by encapsulating single cells in droplets, wherein the droplets further contain reagents for amplifying and sinking native pairings of heavy and light chain variable domain amplicons from single encapsulated cells; lysing the single encapsulated cells; and generating the encapsulated natively-paired scFv amplicons, wherein each scFv amplicon comprises a native pairing of heavy and light chain variable domain amplicons.
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