A METHOD FOR PREPARATION OF alpha-GLUCAN
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United States of America Patent
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Issued Date -
May 2, 2024
app pub date -
Apr 8, 2022
filing date -
Mar 1, 2021
priority date (Note) -
Published
status (Latency Note)
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Abstract
A production method of low molecular weight α-glucan includes: inoculating an activated Leuconostoc mesenteroides in a 5 L fermentor at a 10% inoculum. Fermentation broth is placed in the fermentor at an initial pH of 6.8-7.0, temperature of 25° C. to 28° C., stirring speed at 120 r/min, and fermented for 20-40 hours. Dextranase is added after 5-30 hours of fermentation at a dosage of 1/10,000 to 5/10,000 by volume. The molecular weight of α-glucan is controlled within 10000D by the amount of enzyme added, and the total fermentation process is about 20-40 hours. After the reaction is terminated, the fermentation liquid is concentrated and dried to prepare dietary fiber products with a molecular weight of 500-5000D. The viscosity of the fermentation liquid and concentration of α-glucan in the fermentation liquid may be reduced to promote the forward reaction, accelerate the sucrose conversion rate and increase the product yield.
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