CELL CULTURE METHOD, CELL CULTURE KIT, AND CELL CULTURE SYSTEM
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United States of America Patent
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Issued Date -
Apr 18, 2024
app pub date -
Dec 21, 2023
filing date -
Jun 25, 2021
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Abstract
A culture vessel includes at least a first port used for medium supply and a second port used for medium discharge, and A medium supplying vessel serves as a mixing vessel for adjusting a concentration of A new medium to be supplied to the culture vessel. A cell culture method includes: transferring a portion of a used medium in the culture vessel from the culture vessel to the mixing vessel; creating a concentration-adjusted medium by mixing the new medium and the portion of the used medium in the mixing vessel; and filling the culture vessel with the concentration-adjusted medium by discharging a remaining used medium from the culture vessel while the concentration-adjusted medium is transferred from the mixing vessel to the culture vessel.
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