Peptide Synthesis Processes
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United States of America Patent
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Issued Date -
Mar 14, 2024
app pub date -
Aug 24, 2023
filing date -
Aug 26, 2022
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Abstract
A process for deprotecting a protected amino acid during solid phase peptide synthesis (SPPS) includes removing a protecting group of a protected amino acid in a reaction vessel with a deprotecting base present in the reaction vessel in an amount greater than zero to about 5 vol %, based on the total volume (100 vol %) of a deprotection reaction mixture in the reaction vessel. At least a portion of the deprotecting base evaporates into an upper interior portion of the reaction vessel (e.g., the headspace) during the removing step. The process also includes directing an inert gas through the reaction vessel to remove evaporated deprotecting base from the interior of the reaction vessel (e.g., from the headspace) during the step of removing the protecting group.
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