METHOD FOR CONSTRUCTING MULTIPLEX PCR LIBRARY FOR HIGH-THROUGHPUT TARGETED SEQUENCING
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Mar 7, 2024
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Dec 31, 2021
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Dec 31, 2020
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Abstract
A method for constructing a multiplex PCR library for high-throughput targeted sequencing: first acquiring a targeted DNA product by means of a high-specificity multiplex PCR reaction, and then digesting with a specific endonuclease, such that a specific molecular barcode is produced at the tail end of the PCR product; thus, the library construction process is more efficient, and the accuracy and sequencing depth of the obtained data are also ensured.
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