BIOSYNTHESIS OF VANILLIN FROM ISOEUGENOL
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United States of America Patent
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Feb 15, 2024
app pub date -
Dec 17, 2021
filing date -
Dec 18, 2020
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The present disclosure generally relates to the production of natural vanillin by bioconversion using isoeugenol as the substrate. More specifically, the present methods utilize a fungal isoeugenol monooxygenase encoded by VpIEM gene from Violaceomyces palustris to catalyze the bioconversion of isoeugenol to vanillin, which can be carried out in a cellular system (e.g., bacteria or yeasts) or in an enzymatic reaction mixture without a cellular system.
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