D-beta-HYDROXYBUTYRATE DEHYDROGENASES (BHBDhs) AND THEIR USE FOR KETONE MONITORING
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Nov 30, 2023
app pub date -
May 24, 2023
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May 24, 2022
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Abstract
Engineered D-β-hydroxybutyrate dehydrogenases (BHBDhs) are disclosed. In some embodiments, the BHBDhs are derived from enzymes which are putative homologues of A. faecalis-derived BHBDh, including from thermophilic bacteria and radiation-resistance bacteria. These BHBDhs may also comprise additional mutations to increase their catalytic activity. Further, BHBDhs modified to include an NAD-binding loop, so as to tightly bind an NAD+ co-factor, are also disclosed.
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