CLEAVABLE CYCLIC LOOP NUCLEOTIDES FOR NANOPORE SEQUENCING
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United States of America Patent
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Nov 9, 2023
app pub date -
May 3, 2023
filing date -
May 4, 2022
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Abstract
In one aspect, the disclosed technology relates to nanopore sequencing with a polynucleotide comprising a plurality of nucleotides, wherein each nucleotide comprises a linker construct between two positions of the nucleotide, wherein the linker construct optionally comprises a reporter moiety corresponding to the identity of the nucleotide, and wherein the linker construct is a part of the cleavable cyclic loop nucleotide comprising a cleavable site. In some embodiments, the nucleotides further comprise arresting constructs for slowing or halting the polynucleotide translocation through a nanopore.
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