METHODS OF IDENTIFYING MARKERS OF GRAFT REJECTION
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United States of America Patent
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Issued Date -
Nov 2, 2023
app pub date -
Jun 4, 2021
filing date -
Jun 5, 2020
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The invention relates to polynucleotide probes, with each polynucleotide probe comprising two perfectly complementary strands. In some embodiments, each one of the strands comprises, in a 5′ to 3′ direction, a) a first target hybridization sequence, b) a first digital tag sequence, c) a first Halo barcode sequence, d) a first Halo amplification primer sequence, e) a reverse second Halo amplification primer sequence, f) a reverse second Halo barcode sequence, g) a reverse second digital tag sequence, and h) a reverse second target hybridization sequence. The invention also relates to methods of using these novel probes in to determine the levels of a minor population of DNA amongst a mixture of DNA from two different sources.
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