METHODS, COMPOSITIONS, AND KITS FOR DETECTING ALLELIC VARIANTS
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United States of America Patent
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Issued Date -
Oct 12, 2023
app pub date -
Dec 6, 2022
filing date -
Dec 17, 2008
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Abstract
In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).
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