Method of Producing Proteins Using a Trichoderma Fungus Strain in Which the CEL1A Gene is Invalidated
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United States of America Patent
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Sep 28, 2023
app pub date -
Jun 21, 2021
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Jun 22, 2020
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Abstract
The invention relates to the various uses of a fungus strain which belongs to the Trichoderma genus and in which the cel1a gene has been knocked out. The invention relates in particular to a method of producing proteins by a fungus strain which belongs to the Trichoderma genus and in which the cel1a gene has been knocked out, comprising at least two steps:
- a first step of growth in a batch phase in the presence of at least one carbon growth substrate, anda second step of producing proteins in a fed-batch phase in the presence of a composition comprising at least lactose and a second sugar, the lactose content in the composition representing approximately between 0 and 30% by weight of the total sugar content of the composition, in particular between 5 and 30%.
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