METHODS AND APPARATUS FOR SIMULTANEOUSLY DETECTING A LARGE RANGE OF PROTEIN CONCENTRATIONS

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United States of America Patent

APP PUB NO 20230272003A1
SERIAL NO

18301858

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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Some embodiments described herein relate to a method that includes separating an analyte-containing sample via electrophoresis in a capillary. The capillary is loaded with a chemiluminescence agent, such as luminol, that is configured to react with the analyte (e.g., HRP-conjugated proteins) to produce a signal indicative of a concentration and/or quantity of analyte at each location along the length of the capillary. A first image of the capillary containing the analytes and the chemiluminescence agent is captured over a first period of time. A second image of the capillary containing the analytes and the chemiluminescence agent is captured over a second, longer, period of time. A concentration and/or quantity of a first population of analytes at a first location is determined using the first image, and a concentration and/or quantity of a second population of analytes at a second location is determined using the second image.

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Patent Owner(s)

Patent OwnerAddress
PROTEINSIMPLE3001 ORCHARD PKWY SAN JOSE CA 95134

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
SWARTZ, Kenneth Los Gatos, US 4 23
XU, Hui Dublin, US 440 2426

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