TRANSPARENTIZING PRETREATMENT METHOD OF BIOLOGICAL SAMPLE HAVING SIZE OF AT MOST 1 MM, AND TRANSPARENTIZING METHOD OF BIOLOGICAL SAMPLE INCLUDING SAME
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United States of America Patent
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Issued Date -
Jul 27, 2023
app pub date -
May 28, 2021
filing date -
Jun 16, 2020
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Abstract
A clearing pretreatment method of a biological sample having a size of at most 1 mm, that is, a spheroid or organoid, according to the present invention uses a phosphate-buffered saline (PBS) rather than a conventional sucrose solution as a pretreatment solution, thus solving the problem in which the spheroid or organoid floats above the surface of water due to the density difference such that the structure of the sample is damaged. Therefore, there is an effect in that the spheroid or organoid can be transparentized while maintaining the original shape thereof, thus making it possible to image deep parts.
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