METHODS OF CAPTURING CELL-FREE METHYLATED DNA AND USES OF SAME
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United States of America Patent
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Jun 29, 2023
app pub date -
Dec 2, 2022
filing date -
May 3, 2016
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Abstract
There is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 mg of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.
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