METHOD FOR AMPLIFYING NUCLEOTIDE SEQUENCE, AND SEQUENCE DETERMINATION
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United States of America Patent
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Issued Date -
Jun 22, 2023
app pub date -
Aug 14, 2020
filing date -
Aug 20, 2019
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Abstract
The present invention provides a method for amplifying a sequence adjacent to a specific sequence, comprising the steps of: annealing a first forward primer to the specific sequence to synthesize a complementary strand; sequentially polymerically adding a first deoxynucleotide and a second deoxynucleotide to a 3′-end of the complementary strand; annealing a first reverse primer to a binding site between the 3′-end of the complementary strand and a polydeoxynucleotide strand composed of the first deoxynucleotide to synthesize a double-stranded DNA; performing a PCR with the double-stranded DNA as a template by using a second forward primer complementary to the specific sequence and a first reverse primer; and further performing a PCR by using a third forward primer complementary to the specific sequence and a second reverse primer.
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