COMPOSITION, REACTION LIQUID AND METHOD FOR IMPROVING QPCR TEST PERFORMANCE, AND USE THEREOF
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United States of America Patent
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Issued Date -
Jun 22, 2023
app pub date -
Sep 28, 2022
filing date -
Apr 23, 2020
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Abstract
A composition for improving the detection performance of fluorescent quantitative PCR. The composition comprises bovine serum albumin, sorbitol, ammonium sulfate, formamide, tetramethylammonium chloride, and at least one of dithiothreitol and betaine. The present invention further relates to a qPCR reaction liquid containing the composition and a preparation method therefor. The composition can improve the sensitivity, specificity, and interference resistance of real-time fluorescent quantitative PCR.
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