NUCLEIC ACID SAMPLE ENRICHMENT AND SCREENING METHODS
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United States of America Patent
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Jun 22, 2023
app pub date -
May 18, 2021
filing date -
May 18, 2020
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Abstract
Described herein are methods for enriching test samples for target nucleic acid molecules for further genetic screening. Methods may comprise isolating nucleic acid from test subjects, preparing nucleic acid libraries wherein the nucleic acid molecules are tagged or barcoded to identify sample of origin, determining fragment size distribution, determining abundance of a target nucleic acid population, calculating numerical offset values to determine amount of libraries to add for fragment size selection, performing fragment size selection, and performing a diagnostic assay on a sample enriched for a target nucleic acid.
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