METHOD FOR PREPARING DIACYLGLYCEROL BY IMMOBILIZED SN-2 LIPASE
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United States of America Patent
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Issued Date -
Jun 15, 2023
app pub date -
Dec 8, 2022
filing date -
Dec 14, 2021
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Abstract
A method for preparing 1,3-diacylglycerol by immobilizing Sn-2 lipase. The present application obtains the Sn-2 lipase adsorbed by the macroporous resin by adsorbing the Sn-2 lipase on the resin, cross-links and immobilizes the Sn-2 lipase, and obtains 1,3-diglycerol by one-step hydrolysis with the lipase. The method improves the utilization times, hydrolysis activity and specificity of the Sn-2 lipase during hydrolysis, and determines the optimal parameters by improving the experimental conditions. This makes up for the shortcomings of immobilization and realizes the one-step hydrolysis of 1,3-diacylglycerol. Under optimal conditions, the hydrolase activity of immobilized lipase CAL-A is 1937.86U/g, and the immobilization rate can reach 94.49%, and has good operational stability and storage stability. Secondly, the DAG content can reach 36.12%, which is obviously superior to the current existing technology, and significantly reduces the production cost and market value of 1,3-DAG, providing favorable value for the industrial production of immobilized enzymes.
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