Screening of Engineered Biocatalysts for Oxyfunctionalization of Olefins
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United States of America Patent
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Issued Date -
May 25, 2023
app pub date -
Sep 8, 2022
filing date -
Nov 22, 2021
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Abstract
A variety of methods and systems for screening biocatalysts are disclosed, including, in one embodiment, a screening method for identifying engineered biocatalysts, including reacting an olefin with water in the presence of an engineered biocatalyst to produce at least a fatty alcohol having from 4 carbons to 24 carbons; reacting at least a portion of the fatty alcohol with oxygen in the present of a fatty alcohol oxidase to produce a fatty aldehyde and hydrogen peroxide, the fatty aldehyde having from 4 carbons to 24 carbons; and measuring activity of the engineered biocatalyst.
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