LUCIFERASE-BASED METHODS FOR DETECTING BACTERIAL AND FUNGAL CELLS AND ASSESSING SUSCEPTIBILITY OF BACTERIAL CELLS TO ANTIBIOTICS
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Apr 13, 2023
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Mar 8, 2021
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Mar 6, 2020
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Abstract
The present invention relates to a method for assessing cell viability of bacterial and fungal cells and to a method for the detection of bacterial and fungal cells with specific enzyme activities. The methods of the present invention rely on the real-time measurement of the level of luminescence signal from a luciferase enzyme directly from a growing culture of bacterial or fungal cells. Furthermore, the present invention relates to a method for assessing susceptibility of bacterial cells to antibiotics by measuring ATP levels using a luciferase assay system.
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