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Abstract

Suggested is method for producing galactooligosaccharides, comprising the following steps:

(i) Providing an aqueous milk sugar solution;(ii) Sterilising the milk sugar solution;(iii) Performing a transgalactosilation of the milk sugars present in the sterilised milk sugar solution of step (ii) by adding at least one beta-galactosidase within its optimum temperature and pH value intervals for a period of at least 30 minutes, obtaining a reaction mixture;(iv) Inhibiting the enzyme mass in the reaction mixture of step (iii), and(v) Confectioning the reaction mixture of step (iv),Wherein(a) The enzyme mass is wholly or partly inhibited by setting a pH value outside the activity optimum of the enzymes;(b) The reaction mixture, together with the inhibited enzyme mass, is subjected to filtration, obtaining a retentate (R1) and a permeate (P1);(c) The inhibited amount of enzymes is separated as a retentate (R1) and is fed back to step (iii); and(d) The permeate (P2) containing the galactooligosaccharides is passed on to step (v).

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First Claim