COMPOSITIONS AND METHODS FOR DETECTION OF OXIDIZABLE ANALYTES
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United States of America Patent
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Dec 22, 2022
app pub date -
Oct 20, 2020
filing date -
Oct 20, 2020
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Abstract
Described herein are compositions that are suitable for use in analyte sensing in biological samples and in medical diagnostics. The compositions include an oxidase capable of oxidizing the analyte of interest to produce hydrogen peroxide, a peroxidase, and a chemical compound, such as a near-infrared fluorescent compound, that is a substrate for the peroxidase. The oxidase, the peroxidase, and the chemical compound are encapsulated by vesicle that includes a lipid or polymeric bilayer, such as liposome and polymersome. The peroxidase catalyzes the oxidation of the chemical compound by hydrogen peroxide. Methods of analyte sensing in biological samples using these compositions, and methods of preparing the compositions are also described.
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