PURIFYING AND POLYMERIZING 3'-BLOCKED NUCLEOTIDES
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United States of America Patent
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Dec 8, 2022
app pub date -
May 19, 2022
filing date -
May 19, 2022
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Abstract
A method for purifying nucleotides is provided, that includes preparing a solution comprising (a) 3′-blocked nucleotides, (b) 3′-OH nucleotides, (c) a polishing polymerase, and (d) a template. The polishing polymerase and the template are used to selectively polymerize the 3′-OH nucleotides and thus reduce a concentration in the solution of the 3′-OH nucleotides relative to the 3′-blocked nucleotides.
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