PRODUCTION AND TRACKING OF ENGINEERED CELLS WITH COMBINATORIAL GENETIC MODIFICATIONS
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United States of America Patent
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Issued Date -
Dec 8, 2022
app pub date -
Feb 7, 2020
filing date -
Feb 7, 2020
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Abstract
Described herein are methods for making genetically modified cells by introducing combinations of genetic variants (designed or random) or constructs (genes or otherwise arbitrary DNA) into a population of cells, and for tracking each variant combination by sequentially building an array of barcodes at a common locus (chromosomal or plasmid), termed the barcode locus. Also described are the cells made by such methods.
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