COMPOSITION AND METHOD FOR RENDERING BIOLOGICAL MATERIAL
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United States of America Patent
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Issued Date -
Nov 10, 2022
app pub date -
Aug 7, 2020
filing date -
Aug 7, 2019
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Abstract
The disclosure provides a clearing composition and a method utilizing the clearing composition for rendering a biological sample transparent. The clearing composition includes a RI matching material, a permeating agent including a surfactant, at least two labeling materials, and a solvent. The sample rendering method includes the steps of: (a) fixing a biological sample with a fixative solution; (b) embedding the biological sample into an embedding material; (c) immersing a biological sample in the clearing composition so the sample is permeated by the cleaning composition; and (d) mounting, by a mounting solution, the permeated biological sample.
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