METHODS FOR MANUFACTURING AN ELECTROCHEMICAL SENSOR FOR EFFECTIVE DIAGNOSTIC OLIGONUCLEOTIDE DETECTION
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United States of America Patent
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Oct 13, 2022
app pub date -
Apr 7, 2022
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Apr 7, 2021
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Abstract
The present invention features methods for manufacturing an electrochemical sensor for detecting a diagnostic target oligonucleotide. The methods described herein provide for an electrochemical sensor with a higher level of coverage of the probes on its surface, thus allowing for more sensitive detection of a target oligonucleotide. The methods may feature first mixing disulfide terminated oligonucleotides having a free thiol moiety at the 3′ end with a gold substrate and subsequently introducing to the gold substrate a composition for reducing thiol moieties to cause the oligonucleotides to bind to the surface of the gold substrate. In some embodiments, the method comprises removing excess thiol and oligonucleotides, which may help with non-competitive binding. In some embodiments, the method comprises rinsing the gold substrate with water and drying with nitrogen.
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